publications
2023
A pentameric TRPV3 channel with a dilated pore
Lansky S, Betancourt JM, Zhang J, Jiang Y, Kim ED, Paknejad N, Nimigean CM, Yuan P, Scheuring S. Nature. 2023 Sep;621(7977):206-214. doi: 10.1038/s41586-023-06470-1.
Calcium-gated potassium channel blockade via membrane-facing fenestrations
Fan C, Flood E, Sukomon N, Agarwal S, Allen TW, Nimigean CM. Nat Chem Biol. 2023 Aug 31. doi: 10.1038/s41589-023-01406-2. Epub ahead of print.
Discrimination between cyclic nucleotides in a cyclic nucleotide-gated ion channel
Pan Y, Pohjolainen E, Schmidpeter PA, Vaiana AC, Nimigean CM, Grubmüller H, Scheuring S. Nat Struct Mol Biol. 2023 Apr;30(4):512-520. doi: 10.1038/s41594-023-00955-3.
Membrane phospholipids control gating of the mechanosensitive potassium leak channel TREK1
Schmidpeter PA, Petroff JT 2nd, Khajoueinejad L, Wague A, Frankfater C, Cheng WW, Nimigean CM, Riegelhaupt PM. Nat Commun. 2023 Feb 25;14(1):1077. doi: 10.1038/s41467-023-36765-w
Ball-and-Chain Inactivation in Potassium Channels
Sukomon N, Fan C, Nimigean CM. Annu Rev Biophys. 2023 May 9;52:91-111. doi: 10.1146/annurev-biophys-100322-072921
2022
Gating intermediates reveal inhibitory role of the voltage sensor in a cyclic nucleotide-modulated ion channel
Gao X, Schmidpeter PA, Berka V, Durham RJ, Fan C, Jayaraman V, Nimigean CM. Nat Commun. 2022 Nov 14;13(1):6919. doi: 10.1038/s41467-022-34673-z
Anionic lipids unlock the gates of select ion channels in the pacemaker family
Schmidpeter PA, Wu D, Rheinberger J, Riegelhaupt PM, Tang H, Robinson CV, Nimigean CM. Nat Struct Mol Biol. 2022 Nov;29(11):1092-1100. doi: 10.1038/s41594-022-00851-2
35 years of channelling potassium ions
Nimigean CM. Nature. 2022 Aug;608(7924):670-672. doi: 10.1038/d41586-022-02163-3
2021
Correlation of membrane protein conformational and functional dynamics
Sanganna Gari RR, Montalvo-Acosta JJ, Heath GR, Jiang Y, Gao X, Nimigean CM, Chipot C, Scheuring S. Nat Commun. 2021 Jul 16;12(1):4363. doi: 10.1038/s41467-021-24660-1
Correlating ion channel structure and function
Schmidpeter PA, Nimigean CM. Methods Enzymol. 2021;652:3-30. doi: 10.1016/bs.mie.2021.02.016
2020
Mechanistic insights into volatile anesthetic modulation of K2P channels
Wague A, Joseph TT, Woll KA, Bu W, Vaidya KA, Bhanu NV, Garcia BA, Nimigean CM, Eckenhoff RG, Riegelhaupt PM.. Elife. 2020 Dec 21;9:e59839. doi: 10.7554/eLife.59839
Prolyl isomerization controls activation kinetics of a cyclic nucleotide-gated ion channel
Schmidpeter PA, Rheinberger J, Nimigean CM. Nat Commun. 2020 Dec 16;11(1):6401. doi: 10.1038/s41467-020-20104-4
An ACE2 Microbody Containing a Single Immunoglobulin Fc Domain Is a Potent Inhibitor of SARS-CoV-2
Tada T, Fan C, Chen JS, Kaur R, Stapleford KA, Gristick H, Dcosta BM, Wilen CB, Nimigean CM, Landau NR. Cell Rep. 2020 Dec 1:108528. doi: 10.1016/j.celrep.2020.108528
Selectivity filter ion binding affinity determines inactivation in a potassium channel
Boiteux C, Posson DJ, Allen TW, Nimigean CM. Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29968-29978. doi: 10.1073/pnas.2009624117
Time-resolved cryo-EM using Spotiton
Dandey VP, Budell WC, Wei H, Bobe D, Maruthi K, Kopylov M, Eng ET, Kahn PA, Hinshaw JE, Kundu N, Nimigean CM, Fan C, Sukomon N, Darst SA, Saecker RM, Chen J, Malone B, Potter CS, Carragher B. Nat Methods. 2020 Sep;17(9):897-900. doi: 10.1038/s41592-020-0925-6
Polyamine block of MthK potassium channels
Nimigean CM. J Gen Physiol. 2020 Jul 6;152(7):e202012614. doi: 10.1085/jgp.202012614
Ball-and-chain inactivation in a calcium-gated potassium channel
Fan C, Sukomon N, Flood E, Rheinberger J, Allen TW, Nimigean CM. Nature. 2020 Apr;580(7802):288-293. doi: 10.1038/s41586-020-2116-0
Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses
Schmidpeter PA, Sukomon N, Nimigean CM. Methods Mol Biol. 2020;2127:191-205. doi: 10.1007/978-1-0716-0373-4_14
2019
Direct binding of phosphatidylglycerol at specific sites modulates desensitization of a ligand-gated ion channel
Tong A, Petroff JT 2nd, Hsu FF, Schmidpeter PA, Nimigean CM, Sharp L, Brannigan G, Cheng WW. Elife. 2019 Nov 14;8. pii: e50766. doi: 10.7554/eLife.50766
Identifying coupled clusters of allostery participants through chemical shift perturbations
Xu Y, Zhang D, Rogawski R, Nimigean CM, McDermott AE. Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2078-2085. doi: 10.1073/pnas.1811168116
Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase
Falzone ME, Rheinberger J, Lee BC, Peyear T, Sasset L, Raczkowski AM, Eng ET, Di Lorenzo A, Andersen OS, Nimigean CM, Accardi A. Elife. 2019 Jan 16;8. pii: e43229. doi: 10.7554/eLife.43229.
2018
Fluorescence Titrations to Determine the Binding Affinity of Cyclic Nucleotides to SthK Ion Channels
Schmidpeter PA, Nimigean CM. Bio Protoc. 2018 Oct 5;8(19). pii: e3041. doi: 10.21769/BioProtoc.3041.
An iris diaphragm mechanism to gate a cyclic nucleotide-gated ion channel
Marchesi A, Gao X, Adaixo R, Rheinberger J, Stahlberg H, Nimigean CM, Scheuring S. Nat Commun. 2018 Sep 28;9(1):3978. doi: 10.1038/s41467-018-06414-8.
Ligand discrimination and gating in cyclic nucleotide-gated ion channels from apo and partial agonist-bound cryo-EM structures
Rheinberger J, Gao X, Schmidpeter PA, Nimigean CM. Elife. 2018 Jul 20;7. pii: e39775. doi: 10.7554/eLife.39775.
Ligand binding and activation properties of the purified bacterial cyclic nucleotide-gated channel SthK
Schmidpeter PA, Gao X, Uphadyay V, Rheinberger J, Nimigean CM. J Gen Physiol. 2018 Jun 4;150(6):821-834. doi: 10.1085/jgp.201812023.
High-Resolution Cryoelectron Microscopy Structure of the Cyclic Nucleotide-Modulated Potassium Channel MloK1 in a Lipid Bilayer
Kowal J, Biyani N, Chami M, Scherer S, Rzepiela AJ, Baumgartner P, Upadhyay V, Nimigean CM, Stahlberg H. Structure. 2018 Jan 2;26(1):20-27.e3. doi: 10.1016/j.str.2017.11.012.
Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies
Posson DJ, Rusinova R, Andersen OS, Nimigean CM. Methods Mol Biol. 2018;1684:223-235. doi: 10.1007/978-1-4939-7362-0_17.
2017
Mechanism of activation at the selectivity filter of the KcsA K+ channel
Heer FT, Posson DJ, Wojtas-Niziurski W, Nimigean CM, Bernèche S. Elife. 2017 Oct 10;6. pii: e25844. doi: 10.7554/eLife.25844.
Identification of a pre-active conformation of a pentameric channel receptor
Menny A, Lefebvre SN, Schmidpeter PA, Drège E, Fourati Z, Delarue M, Edelstein SJ, Nimigean CM, Joseph D, Corringer PJ. Elife. 2017 Mar 15;6. pii: e23955. doi: 10.7554/eLife.23955.
Real-time visualization of conformational changes within single MloK1 cyclic nucleotide-modulated channels
Rangl M, Miyagi A, Kowal J, Stahlberg H, Nimigean CM, Scheuring S. Nat Commun. 2016 Sep 20;7:12789. doi: 10.1038/ncomms12789.
2016
Conformational heterogeneity in closed and open states of the KcsA potassium channel in lipid bicelles
Kim DM, Dikiy I, Upadhyay V, Posson DJ, Eliezer D, Nimigean CM. J Gen Physiol. 2016 Aug;148(2):119-32. doi: 10.1085/jgp.201611602. Epub 2016 Jul 18.
Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating
Kim DM, Nimigean CM. Cold Spring Harb Perspect Biol. 2016 May 2;8(5). pii: a029231. doi: 10.1101/cshperspect.a029231.
2015
Calcium ions open a selectivity filter gate during activation of the MthK potassium channel
Posson DJ, Rusinova R, Andersen OS, Nimigean CM. Nat Commun. 2015 Sep 23;6:8342. doi: 10.1038/ncomms9342.
2014
Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorometric assay
Rusinova R, Kim DM, Nimigean CM, Andersen OS. Biophys J. 2014 Mar 4;106(5):1070-8. doi: 10.1016/j.bpj.2014.01.027.
A KcsA/MloK1 chimeric ion channel has lipid-dependent ligand-binding energetics
McCoy JG, Rusinova R, Kim DM, Kowal J, Banerjee S, Jaramillo Cartagena A, Thompson AN, Kolmakova-Partensky L, Stahlberg H, Andersen OS, Nimigean CM. J Biol Chem. 2014 Apr 4;289(14):9535-46. doi: 10.1074/jbc.M113.543389.
Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1
Kowal J, Chami M, Baumgartner P, Arheit M, Chiu PL, Rangl M, Scheuring S, Schröder GF, Nimigean CM, Stahlberg H. Nat Commun. 2014;5:3106. doi: 10.1038/ncomms4106.
2013
Molecular interactions involved in proton-dependent gating in KcsA potassium channels
Posson DJ, Thompson AN, McCoy JG, Nimigean CM. J Gen Physiol. 2013 Dec;142(6):613-24. doi: 10.1085/jgp.201311057.
Preparation of uniformly isotope labeled KcsA for solid state NMR: expression, purification, reconstitution into liposomes and functional assay
Bhate MP, Wylie BJ, Thompson A, Tian L, Nimigean CM, McDermott AE. Protein Expr Purif. 2013 Oct;91(2):119-24. doi: 10.1016/j.pep.2013.07.013.
The voltage-dependent gate in MthK potassium channels is located at the selectivity filter
Posson DJ, McCoy JG, Nimigean CM. Nat Struct Mol Biol. 2013 Feb;20(2):159-66. doi: 10.1038/nsmb.2473.
2012
Structural correlates of selectivity and inactivation in potassium channels
McCoy JG, Nimigean CM. Biochim Biophys Acta. 2012 Feb;1818(2):272-85. doi: 10.1016/j.bbamem.2011.09.007.
2011
Origins of ion selectivity in potassium channels from the perspective of channel block
Nimigean CM, Allen TW. J Gen Physiol. 2011 May;137(5):405-13. doi: 10.1085/jgp.201010551.
Mechanism for selectivity-inactivation coupling in KcsA potassium channels
Cheng WW, McCoy JG, Thompson AN, Nichols CG, Nimigean CM. Proc Natl Acad Sci U S A. 2011 Mar 29;108(13):5272-7. doi: 10.1073/pnas.1014186108.
Non-vesicular transfer of membrane proteins from nanoparticles to lipid bilayers
Banerjee S, Nimigean CM. J Gen Physiol. 2011 Feb;137(2):217-23. doi: 10.1085/jgp.201010558.
2010
Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB
Tanabe M, Nimigean CM, Iverson TM. Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6811-6. doi: 10.1073/pnas.0912115107.
2009
Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore
Thompson AN, Kim I, Panosian TD, Iverson TM, Allen TW, Nimigean CM. Nat Struct Mol Biol. 2009 Dec;16(12):1317-24. doi: 10.1038/nsmb.1703
2008
Molecular mechanism of pH sensing in KcsA potassium channels
Thompson AN, Posson DJ, Parsa PV, Nimigean CM. Proc Natl Acad Sci U S A. 2008 May 13;105(19):6900-5. doi: 10.1073/pnas.0800873105
2007
The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution
Chiu PL, Pagel MD, Evans J, Chou HT, Zeng X, Gipson B, Stahlberg H, Nimigean CM. Structure. 2007 Sep;15(9):1053-64. doi: 10.1016/j.str.2007.06.020
Ligand binding and activation in a prokaryotic cyclic nucleotide-modulated channel
Nimigean CM, Pagel MD. J Mol Biol. 2007 Aug 31;371(5):1325-37. doi: 10.1016/j.jmb.2007.06.030
2006
A radioactive uptake assay to measure ion transport across ion channel-containing liposomes
Nimigean CM. Nat Protoc. 2006;1(3):1207-12. doi: 10.1038/nprot.2006.166
Calcium-dependent gating of MthK, a prokaryotic potassium channel
Zadek B, Nimigean CM. J Gen Physiol. 2006 Jun;127(6):673-85. doi: 10.1085/jgp.200609534
2004
A cyclic nucleotide modulated prokaryotic K+ channel
Nimigean CM, Shane T, Miller C. J Gen Physiol. 2004 Sep;124(3):203-10. doi: 10.1085/jgp.200409133
2003
Electrostatic tuning of ion conductance in potassium channels
Nimigean CM, Chappie JS, Miller C. Biochemistry. 2003 Aug 12;42(31):9263-8. doi: 10.1021/bi0348720
2002
Slo1 tail domains, but not the Ca2+ bowl, are required for the beta 1 subunit to increase the apparent Ca2+ sensitivity of BK channels
Qian X, Nimigean CM, Niu X, Moss BL, Magleby KL. J Gen Physiol. 2002 Dec;120(6):829-43. doi: 10.1085/jgp.20028692
Na+ block and permeation in a K+ channel of known structure
Nimigean CM, Miller C. J Gen Physiol. 2002 Sep;120(3):323-35. doi: 10.1085/jgp.20028614
2000
Functional coupling of the beta(1) subunit to the large conductance Ca(2+)-activated K(+) channel in the absence of Ca(2+). Increased Ca(2+) sensitivity from a Ca(2+)-independent mechanism
Nimigean CM, Magleby KL. J Gen Physiol. 2000 Jun;115(6):719-36. doi: 10.1085/jgp.115.6.719
1999
Ca2+-dependent gating mechanisms for dSlo, a large-conductance Ca2+-activated K+ (BK) channel
Moss BL, Silberberg SD, Nimigean CM, Magleby KL. Biophys J. 1999 Jun;76(6):3099-117. doi: 10.1016/S0006-3495(99)77462-X
The beta subunit increases the Ca2+ sensitivity of large conductance Ca2+-activated potassium channels by retaining the gating in the bursting states
Nimigean CM, Magleby KL. J Gen Physiol. 1999 Mar;113(3):425-40. doi: 10.1085/jgp.113.3.425